Journal:

Article Title: Estradiol-17? Stimulates Specific Receptor and Endogenous Nitric Oxide-Dependent Dynamic Endothelial Protein S -Nitrosylation: Analysis of Endothelial Nitrosyl -Proteome

doi: 10.1210/en.2009-1356

Figure Lengend Snippet: CyDye switch, 2D-DIGE analysis of nitrosyl-proteomes in control (Ctl) and estrogen-treated HUVEC. HUVEC were treated with or without E2 (10 nm) for 20 min. Total cell extracts (50 μg/treatment) were incubated with ascorbate. Control samples were labeled with Cy3 (green), and E2-treated samples were labeled with Cy5 (red). The samples were then mixed (total 100 μg) and then separated on analytical 2D-DIGE. The gel was scanned with a fluorescence scanner in green (Cy3, 548/560 nm) and red (Cy5, 641/660 nm) channels. A merged fluorescence image shown represents one of three separate experiments using cells from different placentas. Red, green, and yellow spots represent nitrosoproteins that were increased, decreased or unchanged by E2, respectively. The spots circled and numbered represents 58 nitrosoproteins as listed in Table 1​1​,, which were identified by MALDI-TOF and MS/MS. The lower black and white images shown represent fluorescent signals obtained from the red and green channels of one of three experiments.

Article Snippet: Protein identification by matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF) and tandem MS (mass spectrometry) (MS/MS) Protein identification was performed by Applied Biomics, Inc. After analyses of the 2D-DIGE image, selected spots of interest (based on intensity and visibility) were picked up.

Techniques: Control, Incubation, Labeling, Fluorescence, Tandem Mass Spectroscopy

Journal: Frontiers in Veterinary Science

Article Title: Epizootic Yersinia enterocolitica in captive African green monkeys ( Chlorocebus aethiops sabaeus )

doi: 10.3389/fvets.2022.922961

Figure Lengend Snippet: Summary of gross lesions by organ system in African green monkeys infected with Yersinia enterocolitica ^a .

Article Snippet: Any growth after 24 h was submitted to MIDI Labs (Newark, DE) for Y. enterocolitica sub-culture and identification using Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry.

Techniques: Infection

Journal: Frontiers in Veterinary Science

Article Title: Epizootic Yersinia enterocolitica in captive African green monkeys ( Chlorocebus aethiops sabaeus )

doi: 10.3389/fvets.2022.922961

Figure Lengend Snippet: Summary of histologic lesions by organ system in African green monkeys infected with Yersinia enterocolitica ^a .

Article Snippet: Any growth after 24 h was submitted to MIDI Labs (Newark, DE) for Y. enterocolitica sub-culture and identification using Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry.

Techniques: Infection

Journal: Frontiers in Veterinary Science

Article Title: Epizootic Yersinia enterocolitica in captive African green monkeys ( Chlorocebus aethiops sabaeus )

doi: 10.3389/fvets.2022.922961

Figure Lengend Snippet: Immunohistochemistry of selected Yersinia enterocolitica positive and age-matched negative control tissues ^a .

Article Snippet: Any growth after 24 h was submitted to MIDI Labs (Newark, DE) for Y. enterocolitica sub-culture and identification using Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry.

Techniques: Immunohistochemistry, Negative Control

Journal: Frontiers in Veterinary Science

Article Title: Epizootic Yersinia enterocolitica in captive African green monkeys ( Chlorocebus aethiops sabaeus )

doi: 10.3389/fvets.2022.922961

Figure Lengend Snippet: Immunohistochemistry for myeloperoxidase (MPO)-positive neutrophils, vervets, multiple organs. (A) Y. enterocolitica- positive vervet, mesenteric lymph node, case 6. Neutrophils are present within the medullary sinus of a mesenteric lymph node adjacent to the affected colon. (B) Control vervet, mesenteric lymph node. Neutrophils are rarely observed within the control lymph node. (C) Y. enterocolitica- positive vervet, placenta, case 20. MPO-positive neutrophils are abundant within areas of placental necrosis. (D) Control vervet, placenta. Few neutrophils are present within the placenta, and often associated with physiologically normal areas of coagulative necrosis and mineral. (E) Y. enterocolitica- positive vervet, colon, case 3. MPO-positive neutrophils are prominent within mucosal lesions. Neutrophils are also present within veins and lymphatic vessels of the muscularis mucosa. (F) Control vervet, colon. Neutrophils within the control colon are rare and mainly within the lamina propria. (G) Y. enterocolitica- positive vervet, lung, case 1. Extravasated neutrophils are abundant within lesions of the lung. (H) Control vervet, lung. MPO-positive neutrophils are present only within blood vessels of the control lung.

Article Snippet: Any growth after 24 h was submitted to MIDI Labs (Newark, DE) for Y. enterocolitica sub-culture and identification using Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry.

Techniques: Immunohistochemistry, Control

Journal: Frontiers in Veterinary Science

Article Title: Epizootic Yersinia enterocolitica in captive African green monkeys ( Chlorocebus aethiops sabaeus )

doi: 10.3389/fvets.2022.922961

Figure Lengend Snippet: Immunohistochemistry for HAM56, vervets, multiple organs. (A) Y. enterocolitica- positive vervet, mesenteric lymph node, case 6. About 40% of the cells within the medullary sinus are HAM56-positive macrophages. This lymph node was adjacent to a colonic lesion. (B) Control vervet, mesenteric lymph node. A control lymph node contains few HAM56-positive macrophages (<10%). (C) Y. enterocolitica- positive vervet, placenta, case 20. Abundant HAM56-positive macrophages are present in fetal villi and also within maternal blood. (D) Control vervet, placenta. HAM56-positive macrophages are absent in the control placenta. (E) Y. enterocolitica- positive vervet, colon, case 3. HAM56-positive macrophages are present adjacent to necrosis. (F) Control vervet, colon. Macrophages are distributed throughout the colonic mucosa. (G) Y. enterocolitica- positive vervet, lung, case 1. Macrophages are prominent in alveoli at the periphery of necrotic lesions. (H) Control vervet, lung. HAM56-positive macrophages are scattered within alveolar walls.

Article Snippet: Any growth after 24 h was submitted to MIDI Labs (Newark, DE) for Y. enterocolitica sub-culture and identification using Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry.

Techniques: Immunohistochemistry, Control

Journal: Frontiers in Veterinary Science

Article Title: Epizootic Yersinia enterocolitica in captive African green monkeys ( Chlorocebus aethiops sabaeus )

doi: 10.3389/fvets.2022.922961

Figure Lengend Snippet: Immunohistochemistry for CD11c, vervets, multiple organs. (A) Y. enterocolitica- positive vervet, mesenteric lymph node, case 6. Approximately 20% of cells within the lymph node medullary sinus stain positively for CD11c. (B) Control vervet, mesenteric lymph node. CD11c-positive antigen-presenting cells (APCs) make up about 80% of the cells within the cortex and medullary sinuses of the lymph node. (C) Y. enterocolitica- positive vervet, placenta, case 20. CD11c-positive APCs are centered on areas of necrosis and are also increased in maternal blood. (D) Control vervet, placenta. CD11c-positive cells are rare within the control placenta. (E) Y. enterocolitica- positive vervet, colon, case 3. CD11c-positive cells are prevalent within lesions. (F) Control vervet, colon. Two CD11c-positive APCs are present within the colonic mucosa. (G) Y. enterocolitica- positive vervet, lung, case 1. Abundant CD11c-positive cells are found within necrotic lesions. (H) Control vervet, lung. CD11c-positive cells are numerous but confined to the interstitial space of the control lung.

Article Snippet: Any growth after 24 h was submitted to MIDI Labs (Newark, DE) for Y. enterocolitica sub-culture and identification using Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry.

Techniques: Immunohistochemistry, Staining, Control

Journal: Frontiers in Veterinary Science

Article Title: Epizootic Yersinia enterocolitica in captive African green monkeys ( Chlorocebus aethiops sabaeus )

doi: 10.3389/fvets.2022.922961

Figure Lengend Snippet: Immunohistochemistry for CD3, vervets, multiple organs. (A) Y. enterocolitica- positive vervet, mesenteric lymph node, case 6. Few CD3-positive cells are present within the lymph node medulla, with the remaining cells confined to perifollicular regions of the cortex. (B) Control vervet, mesenteric lymph node. Greater than 90% of cells within the lymph node are CD3-positive T cells. (C) Y. enterocolitica- positive vervet, placenta, case 20. CD3-positive T cells are absent in the placenta from a Y. enterocolitica- positive vervet. (D) Control vervet, placenta. CD3-positive T cells are absent from a gestational date-matched control in the placenta. (E) Y. enterocolitica- positive vervet, colon, case 3. CD3-positive T cells are absent from colonic sections as lesions effaced gut-associated lymphoid tissue. (F) Control vervet, colon. CD3-positive lymphocytes are present within gut-associated lymphoid tissue and colonic mucosa. (G) Y. enterocolitica- positive vervet, lung, case 1. CD3-positive T cells are absent in lung sections. (H) Control vervet, lung. T cells are also absent in an age-matched area of control lung.

Article Snippet: Any growth after 24 h was submitted to MIDI Labs (Newark, DE) for Y. enterocolitica sub-culture and identification using Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry.

Techniques: Immunohistochemistry, Control

Journal: Frontiers in Veterinary Science

Article Title: Epizootic Yersinia enterocolitica in captive African green monkeys ( Chlorocebus aethiops sabaeus )

doi: 10.3389/fvets.2022.922961

Figure Lengend Snippet: Immunohistochemistry for CD20, vervets, multiple organs. (A) Y. enterocolitica- positive vervet, mesenteric lymph node, case 6. CD20-positive B cells are present in follicles and the medullary sinus. (B) Control vervet, mesenteric lymph node. CD20-positive cells are most prevalent in the cortical follicles. (C,D) CD20-positive cells are rare in the placenta from a Y. enterocolitica- positive vervet (C, case 20) and in the control vervet (D) . (E) Y. enterocolitica- positive vervet, colon, case 3. CD20-positive B cells are present in the colonic mucosa in areas of presumed gut-associated lymphoid tissue that are mostly effaced by necrosis and suppurative inflammation. (F) Control vervet, colon. CD20-positive B lymphocytes are abundant within gut-associated lymphoid tissue and rare in colonic mucosa. (G) Y. enterocolitica- positive vervet, lung, case 20. Few CD20-positive B cells lie adjacent to areas of necrosis. (H) Control vervet, lung. B cells are more prevalent and scattered throughout the alveolar walls of the control lung.

Article Snippet: Any growth after 24 h was submitted to MIDI Labs (Newark, DE) for Y. enterocolitica sub-culture and identification using Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry.

Techniques: Immunohistochemistry, Control